amma Radiation: Physical Elicitor for In vitro Culture Techniques of Cajanus cajan.

Gamma rays are very important in mutation breeding and in in vitro mutagenesis in order to develop required features of plants and increase the genetic variability. Cajanus cajan when subjected to absorbed doses 30 Gy, 50 Gy, 100 Gy, 150 Gy and 200 Gy showed a direct corelation between callus induction, regeneracy frequency and absorbed doses of gamma radiation as compared to control. Gamma irradiation resulted in the induction of autonomous growth in callus, which led to the formation of callus tumors resembling the shape of crown gall tumors. Gamma irradiation in the present study proved to be an important tool in increasing the breeding efficiency, and regeneration frequency, especially that of the recalcitrant varieties.

Significance of anti-HBc screening of blood donors & its association with occult hepatitis B virus infection: Implications for blood transfusion.

Background & objective: Expansions of blood donor screening and improved laboratory detection of viral markers have remarkably reduced the risk for infection with transfusion-transmitted viruses. This study was aimed to evaluate the presence of anti-HBc and to determine the presence or absence of HBV DNA in the serum samples from HBsAg negative, anti-HBc positive blood donors in a tertiary care hospital blood bank from Delhi. Methods: A total of 2175 HBsAg negative, first time volunteer blood donors were included in the study from blood bank, Lok Nayak Hospital, New Delhi. The blood specimens from all these subjects were evaluated for anti-HBV-core antigen (anti-HBc) serology, anti-HBV-surface antigen (anti-HBs) titres and HBeAg. The presence of HBV DNA was evaluated by testing, through polymerase chain reaction (PCR) techniques. Results: Of the 2175 HBsAg negative voluntary blood donors, 413 (19.8%) were tested to be positive for anti-HBc alone. Of these, 153 (group-I) were anti-HBs negative whereas group-II comprises a total of 260 anti-HBs positive cases i.e. 89 out of 413 had anti-HBs titres of 10-99 IU/l and the remaining 171 had anti-HBs titres of 100-500 IU/l. HBV DNA was detected in 7.5 per cent anti-HBc positive samples irrespective of anti-HBs status. Interpretation & conclusions: Our results showed that 18.9 per cent of our donor population was anti-HBc reactive, and hence inclusion of anti-HBc testing will lead to a high discard rate. The presence of HBV DNA in fairly high percentage of anti-HBc positive samples highlighted the need for a stringent and better screening system to prevent occult HBV infection.

Antituberculosis drug-induced hepatitis: risk factors, prevention and management.

Apart from infectious or viral hepatitis, other most common non-infectious causes of hepatitis are alcohol, cholestatic, drugs and toxic materials. The most common mode that leads to liver injuries is antituberculosis drug-induced hepatitis. The severity of drug-induced liver injury varies from minor nonspecific changes in hepatic structure to fulminant hepatic failure, cirrhosis and liver cancer. Patients receiving antitubercular drug frequently develop acute or chronic hepatitis. The time required for the metabolites to reach hepatotoxic levels is much earlier with isoniazid plus rifampicin treatment than isoniazid alone and this has been shown to be synergistic rather than additive. Antituberculosis drug (ATT)-inducible cytochrome P-4502E1 (CYP2E1) is constitutively expressed in the liver. Recent studies show that polymorphism of the N-acetyltransferase 2 (NAT2) genes and glutathione-S-transferase (GST) are the major susceptibility risk factors for ATT-induced hepatitis. The hepatic NAT and GST are involved in the metabolism of several carcinogenic arylamines and drugs. The NAT2 enzyme has a genetic polymorphism in human. N-acetyltransferase 2 genes (NAT2) have been identified to be responsible for genetic polymorphism of slow and rapid acetylation in humans. Slow acetylators of NAT2 prove to develop more severe hepatotoxicity than rapid acetylators making it a significant risk factor. Deficiency of GST activity, because of homozygous null mutations at GSTM1 and GSTT1 loci, may modulate susceptibility to drug and xenobiotic-induced hepatotoxicity. Polymorphisms at GSTM1, GSTT1 and NAT2 loci had been linked to various forms of liver injury, including hepatocellular carcinoma.

Moalejat-e-hindi: a compilation of ăyurvĕdic formulations tested by Nizam--III of Hyderabad.

This Indian Institute of History of Medicine possesses more than 100 medical manuscripts (paper) in Arabic, Persian and Urdu languages. One of the rare manuscripts is known as "Moalejat-e-Hindi", its specialty is that it is on ăyurvĕda in Persian. It contains shlokas in transliterated form. It contains three sections. It has been compiled by the order of Nizam-III of Hyderabad.

Ayurvedic literature in Urdu part-III.

The present article is the continuation of the second part of the previous work published with the same title in the Bulletin of Indian Institute of History of medicine, Vol. XXIX. No. 2 in 1999. This work has ben initiated to introduce the books written in recent past, the period in which Urdu language had a prominent role in preserving the knowledge of old arts and sciences.

Lessor known ayurvedic physicians from an Urdu book Rumoozul Atibba: part II.

Rumoozul Atibba, a rare Urdu book compiled by Hakim Fairozuddin is in two Volumes. It is published by Darul Kutub Rafiqul Atibba, printed in "Rifah-e-Aam press in 1913 at Lahore which contains short biographies of Ayurvedic and Unani physicians. It has been compiled to disclose the tested formulae hidden in the minds of eminent scholars of Ayurveda and Unani with their life sketches.The selection of this work has been aimed to enlight the Ayurvedic History prevailed in different languages. Twenty biographies of Ayurvedic Physicians were in first volume of 'Rumuzul Atibba', which were published prior to this article (Bulletin of Indian Institute of History of Medicine. Vol XXVI (1996).This article contains only three biographies of Ayurvedic physicians.

Translation of the twelfth chapter of "Uyoonul Anba Fi Tabaqatil Atibba".

This is a translation of an Arabic book "Uyoonul Anba Fi Tabaqatil Atibba" compiled by Ibn-e-Abi Useibia, the famous writer of Abbaside's period of Baghdad in 13th century A.D. This book is in two volumes comprising of 15 chapters dealing with 388 biographies of world known physicians. Looking into the authenticity and importance of this work, the translation of its 12th chapter which is on Indian physicians is being submitted here in this article form. This portion provides information with regard to the Indian physicians, the way of their approach to Baghdad, their miraculous treatments and the literary works etc.

Alauddin Qarshi: a commentator of many classical treatises.

This article deals with the life and work of a commentator of six authentic works. He was a discoverer of pulmonary circulation of blood. He described it more or less accurately, almost three centuries before Harvey (1578-1657). The views of medical historians about this scholar have been given in this article.

Ayurveda during Abbasid's period.

This is a historical paper which deals with a brief account of Abbasid's period. In this article the existence of Ayurveda in Arab countries, arrival of Ayurvedic physicians to Baghdad, their eminence, authenticity and literary additions in medical field has been studied and presented.

Ayurvedic literature in Urdu (Part-II).

This article is a second part of previous work published with the same title in this Bulletin Vol. 28, 1998, P. nos. 151-158. It contains brief notes on six rare Ayurvedic books in Urdu. It provides two types of information, i.e. (i) Introduction to the books studied and (2) Literary history of Ayurveda of that period in which Urdu language had a prominent role in propagation of Arts and Science. It gives an idea for seeking information on these lines regarding the Ayurvedic works in Arabic and Persian also.

Ayurvedic literature in Urdu.

Urdu is a distinct literary language began to develop in India during Mughal empire. Lots of people read, write and speak, this language in Indian subcontinent. This brief article will shed light on the seven rare books in Urdu compiled and published in the period from 19th century to the first half of the current century. Some books may not have high value but the struggle of the scholars, is appreciable.

A rare report of Nizam Ayurvedic Mobile Clinic.

This article is a brief account of a rare Urdu booklet entitled 'Report Nizam Ayurvedic Safari Dawakhana'. It is a report of a mobile clinic sent by Nizam VII of Hyderabad to Kumbh Mela at Allahabad on a religious occasion in the year 1942, which was printed in the same year. This report sheds light on the history of the sacred place Prayag, its importance and the activities of the mobile clinic with the remarks of eminent personalities who visited the clinic on the occasion.

Hakeem Ali Gilani: a commentator of canon of Avicenna.

Hakim Ali Gilani was a native of Gilan. He was a distinguished physician and medical writer. He wrote commentary on canon of Avicenna. He came to India during Akbar's time and served two Mughal rulers, Akbar and Jahangir. He was praised very much by the medical historians for his vast knowledge, curiosity and intelligence. A brief account of his achievements has been given in this article.

Abul Fatah - a commentator of "Al-Qanoon".

The Commentator of "Al-Qanoon" (Canon of Avicenna) Abul Fatah known as "Maseehuddin" also was a native of Gilan. He migrated to India during Akbar's period (1556-1605 A.D.). The emperor honoured him by confering Sadarat of Delhi, Malwa and Gujarat and bestowed upon him the title of Hasht Sadi. His commentary on the Canon is known as "Fatahi", in which he explained various problems elaborately. He was well versed in anatomy and connoisseur of Prose and Poetry. He had made a good change in Huble-Buble and he was the first who had made the inhailing of smoke less harmful. He died on 20th June, 1580 A.D.

Lesser known Ayurvedic physicians of India from an Urdu book- "Rumoozul Atibba".

In early 20th century some Urdu scholars tried to enrich urdu literature with medical history. They started compiling and publishing the biographies of the contemporary physicians along with their achievements. One of such works "Rumoozul Atibba" a rare book compiled by Hakim Fairozuddin in Urdu, published by 'Darul Kutub Rafiqul Atibba' and printed in 'Rifah-e-Aam Press' at Lahore in the year 1913 A.D. has been taken here. It contains about 150 short biographies of Ayurvedic and Unani physicians, out of which all the twenty biographies of Ayurvedic physicians given in it, have been presented in this article.

Moalejat-E-Hindi.

It is an introductory note on a rare and important medical manuscript, which were compiled in Persian language by the order of the 3rd Nizam of Hyderabad. It contains a number of Ayurvedic formulations, found very effective by him. These preparations were prepared and preserved by the Nizam in his royal store-house. The Sanskrit shiokas found in it are in transliterated form, written in beautiful script.

Chromatid association in acrocentric chromosomes of abnormal sexual development (ASD) cases.

Concordant/discordant associations at chromatid level were compared and found significant (P less than 0.05) in females with primary amenorrhea. This probably suggested that the acrocentric association pattern in this group of ASD and infertility did not follow a random segregation in subsequent cell divisions and that the concordant acrocentric chromosomes have regularly established physical connections with one another, held together for several cell cycles. It could only be speculated that the association of acrocentric chromosome anomalies in some females with abnormal sex chromosomes are due to this reason. In the event that chromosome association has a bearing on chromosome aberrations, the non-random pattern of acrocentric association probably would increase the choice for translocation and non disjunction in the somatic cells in females with primary amenorrhea during ontogenesis.

Frequency of acrocentric association in abnormal sexual development in males and females.

A significant difference (P less than 0.05) was observed in a chi 2 comparison of DD, GG and DG-DI associations between male hypogonads and females with primary amenorrhea. This difference increased still further (P less than 0.01) when only DD and GG associations were compared between males and females with abnormal sexual development (ASD). Similarly, when normal males and females were compared for DI, TRI, TETRA, DD vs GG and DG vs GG acrocentric chromosome associations, a significant difference (P less than 0.05) was again observed. The sex difference was also apparent in TRI and TETRA acrocentric associations both in abnormal and normal sexual development males and females. These results suggested that probably sex difference (may be hormonal) influences the number and/or type of acrocentric chromosomes involved in association between males and females with ASD and also between normal males and females.